SrasV1, Main, Exploration, bibRecord, 003109

Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein.

Identifieur interne : 003109 ( Main/Exploration ); précédent : 003108; suivant : 003110

Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein.

Auteurs : Hongyan Guo [République populaire de Chine] ; Aizhen Guo ; Chong Wang ; Bangfen Yan ; Haisong Lu ; Huanchun Chen

Source :

Research in veterinary science [ 0034-5288 ] ; 2008.

RBID : pubmed:17658563

Descripteurs français

KwdFr :
- Amorces ADN, Amplification de gène, Animaux, Chats, Chiens, Clonage moléculaire, Données de séquences moléculaires, Humains, Peptidyl-Dipeptidase A (génétique), Protéines de la matrice virale (métabolisme), Rats, Souris, Séquence conservée, Séquence d'acides aminés, Virus du SRAS, Viverridae.
MESH :
- génétique : Peptidyl-Dipeptidase A.
- métabolisme : Protéines de la matrice virale.
- Amorces ADN, Amplification de gène, Animaux, Chats, Chiens, Clonage moléculaire, Données de séquences moléculaires, Humains, Rats, Souris, Séquence conservée, Séquence d'acides aminés, Virus du SRAS, Viverridae.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Cats, Cloning, Molecular, Conserved Sequence, DNA Primers, Dogs, Gene Amplification, Humans, Mice, Molecular Sequence Data, Peptidyl-Dipeptidase A (genetics), Rats, SARS Virus, Viral Matrix Proteins (metabolism), Viverridae.
MESH :
- chemical , genetics : Peptidyl-Dipeptidase A.
- chemical , metabolism : Viral Matrix Proteins.
- chemical : DNA Primers.
- Amino Acid Sequence, Animals, Cats, Cloning, Molecular, Conserved Sequence, Dogs, Gene Amplification, Humans, Mice, Molecular Sequence Data, Rats, SARS Virus, Viverridae.

Abstract

Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19- 367 aa was expressed in Escherishia coli. Both Western blotting and ELISA demonstrated that fACE2 could react with SARS-CoV S1 protein as efficiently as ACE2 of Vero E6 cells did.

DOI: 10.1016/j.rvsc.2007.05.011
PubMed: 17658563

Affiliations:

Le document en format XML

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<author><name sortKey="Lu, Haisong" sort="Lu, Haisong" uniqKey="Lu H" first="Haisong" last="Lu">Haisong Lu</name>
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<term>Conserved Sequence</term>
<term>DNA Primers</term>
<term>Dogs</term>
<term>Gene Amplification</term>
<term>Humans</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
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<term>Protéines de la matrice virale (métabolisme)</term>
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<term>Séquence d'acides aminés</term>
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<term>Cats</term>
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<term>Conserved Sequence</term>
<term>Dogs</term>
<term>Gene Amplification</term>
<term>Humans</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
<term>Rats</term>
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<term>Clonage moléculaire</term>
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<term>Rats</term>
<term>Souris</term>
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<front><div type="abstract" xml:lang="en">Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19- 367 aa was expressed in Escherishia coli. Both Western blotting and ELISA demonstrated that fACE2 could react with SARS-CoV S1 protein as efficiently as ACE2 of Vero E6 cells did.</div>
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<name sortKey="Wang, Chong" sort="Wang, Chong" uniqKey="Wang C" first="Chong" last="Wang">Chong Wang</name>
<name sortKey="Yan, Bangfen" sort="Yan, Bangfen" uniqKey="Yan B" first="Bangfen" last="Yan">Bangfen Yan</name>
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Serveur d'exploration SRAS

Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein.

Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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